FAP Knockout cell line(U-87 MG)

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FAP Knockout cell line(U-87 MG) Order

Catalog#

YKO-H314

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

FAP Knockout cell line (U-87 MG)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

FAP

Gene ID

2191

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Accession

CVCL_E1EF(Cellosaurus)

Target region

Knockout region: Exon 8 (based on transcript FAP-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:U-87 MG			STR Info (Cell bank) Cell Line:U-87MG
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	8	11		8	11
D7S820	8	9		8	9
D16S539	12	12		12	12
VWA	15	17		15	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	10	11		10	11
D12S391	18	21
FGA	18	24
D2S1338	20	23
D21S11	28	32.2
D18S51	13	13
D8S1179	10	11
D3S1358	16	17
D6S1043	11	18
PENTAE	7	14
D19S433	15	15.2
PENTAD	9	14
D1S1656	15	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FAP gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-87 MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FAP gene knockout FAP Knockout cell line (U-87 MG) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

FAP Knockout cell line(U-87 MG)

Catalog#

YKO-H314

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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FAP Knockout cell line(U-87 MG)

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